Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance

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Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance.

Methodology was developed to isolate and regenerate protoplasts from the biocontrol fungus Gliocladium virens and to transform them to benomyl resistance with a Neurospora crassa beta-tubulin gene. Southern blots demonstrated that multiple copies of the vector integrated into the chromosomal DNA of stable biotypes but not of abortive transformants. Analysis of nuclear condition in vegetative an...

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Production of Extracellular Proteins by the Biocontrol Fungus Gliocladium virens.

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1990

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.56.10.3052-3056.1990